This report summarizes findings from a systematic review of the nine SLSI Safety Culture Assessment Reports created in 2020, and discusses industry trends in the strengths and gaps related to safety culture practices. These strengths (e.g., employees feel empowered to work safely) and gaps (e.g., a lack of formal safety concern reporting systems) provide insight to and indicators of the status of safety culture in the short line and regional railroad industry. The report demonstrated that SLSI is equipped to help railroads address current industry gaps and assist railroads in developing systems they need for creating a strong safety culture. Lastly, this report highlights new ways for SLSI to continue to serve the rail industry.